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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
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Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.

Journal: BMC Medical Genomics

Article Title: Integrated microarray and multiplex cytokine analyses of Kaposi's Sarcoma Associated Herpesvirus viral FLICE Inhibitory Protein K13 affected genes and cytokines in human blood vascular endothelial cells

doi: 10.1186/1755-8794-2-50

Figure Lengend Snippet: Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.

Article Snippet: The microarray experiment design, setup, and data have been deposited in National Center for Biotechnology Information's Gene Expression Omnibus and are accessible through GEO series accession number GSE16051.

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Microarray